Journal:

Article Title: A defect in bone marrow derived dendritic cell maturation in the nonobesediabetic mouse

doi: 10.1046/j.1365-2249.2001.01473.x

Figure Lengend Snippet: Surface phenotype of bone marrow derived DC. The DC consisting of 90–95% CD11c+ cells were analysed by flow cytometry for the expression of I-A, β2-microglobulin, CD80, CD86 and CD40 (▪) or by staining with secondary antibody alone (□). Two I-A specific mAbs, Ox-6 (shown here) or 10·2.16 were used with identical result. The results are representative of three different experiments carried out three weeks apart.

Article Snippet: The antibodies used in the study were the MHC class II specific rat antimouse I-A k clone MRC Ox-6 (Serotec, Oxford, UK) and anti-I-A g7 clone 10·2.16 (kindly provided by EP Reich, Anergen Inc), rat antimouse CD80 (B7·1) clone IG10 (Pharmingen, San Diego, USA), rat antimouse CD86 (B7·2) clone GL1 (Cambridge Bioscience, UK); rat antimouse CD40 clone 3/23 (Serotec, UK), hamster antimouse CD11c clone N-418 (produced by ICRF, London, UK); FITC-conjugated rabbit antirat Ig (Dako, Ely, UK); FITC-conjugated rabbit anti‐mouse Ig (Dako, Denmark); and mouse anti‐mouse β2-microglobulin clone S19·8 (kindly provided by M Millrain, CSC, UK).

Techniques: Derivative Assay, Flow Cytometry, Expressing, Staining

Journal: iScience

Article Title: Temporal and spatial dynamics of immune cells in spontaneous liver transplant tolerance

doi: 10.1016/j.isci.2023.107691

Figure Lengend Snippet: List of metal-labeled antibodies used for mass cytometry

Article Snippet: Anti-CD40 , Fluidigm , 3161020C.

Techniques:

Journal: iScience

Article Title: Temporal and spatial dynamics of immune cells in spontaneous liver transplant tolerance

doi: 10.1016/j.isci.2023.107691

Figure Lengend Snippet:

Article Snippet: Anti-CD40 , Fluidigm , 3161020C.

Techniques: Transplantation Assay, Recombinant, Staining, Cell Isolation, Software, Cytometry, Flow Cytometry